anti cmyc  (Cell Signaling Technology Inc)

Journal: Neural Regeneration Research

Article Title: Low-level expression of Cmyc in mature neurons: Maintaining neuronal function and preventing neurodegeneration

doi: 10.4103/NRR.NRR-D-24-01367

Figure Lengend Snippet: Cmyc knockout leads to behavioral changes in mice. (A) Open field test trajectory plots of the wild-type C57BL/6 group, control group, Cymc -knockout-10 days group ( Cmyc -KO-10d) ( n = 3), Cmyc knockout-30 days group ( Cmyc -KO-30d) ( n = 3), and Cmyc knockout-60 days group ( Cmyc -KO-60d) ( n = 3). All of the Cmyc -KO mice displayed curved movement trajectories accompanied by a leftward movement tendency. (B) The average total distance traveled of mice in the open field test. (C) The number of left turns within 5 minutes in the open field test. (D) The number of leftward inclinations in 40 iterations of the tail suspension swing test. Data are presented as the mean ± SEM. * P < 0.05, *** P < 0.001, **** P < 0.0001, vs. WT mice (one-way analysis of variance followed by Tukey’s post hoc test). KO: Knockout; WT: Wild-type.

Article Snippet: Cmyc flox/– heterozygous mice and wild type (WT) mice were procured from Shanghai Model Organisms Center in Shanghai, Inc. (License No. SCXK (Yu) 2020-0005).

Techniques: Knock-Out, Control, Suspension

Journal: Neural Regeneration Research

Article Title: Low-level expression of Cmyc in mature neurons: Maintaining neuronal function and preventing neurodegeneration

doi: 10.4103/NRR.NRR-D-24-01367

Figure Lengend Snippet: Cmyc knockout decreases the expression levels of DAT and TH in mice. (A) Immunofluorescence staining images of D1, D2, DAT in the striatum and TH in the substantia nigra pars compacta (SNpc) of WT, Control, and Cmyc -KO mice. From left to right showing green fluorescence signal: D1R, D2R, DAT and TH. The expression of DAT was decreased 10 days after Cmyc knockout ( n = 3), and dopaminergic neurons were lost 60 days after Cmyc knockout ( n = 3) in Cmyc -KO mice (scale bar: 400 μm). (B) TUNEL staining of WT mice and Cmyc knockout mice. Neuronal apoptosis occurred in the substantia nigra 60 days after Cmyc knockout. Scale bars: 200 μm for low magnification images and 40 μm for high magnification images. (C–E) D1R, D2R, and DAT fluorescence intensity. (F) The number of dopaminergic neurons (TH + cells). ** P < 0.01, **** P < 0.0001, vs . WT (one-way analysis of variance followed by Tukey’s post hoc test). Data are presented as the mean ± SEM. D1R: Dopamine D1 receptor; D2R: dopamine D2 receptor; DAT: dopamine transporter; KO: knockout; ns: not significant; TH: tyrosine hydroxylase; WT: wild-type.

Article Snippet: Cmyc flox/– heterozygous mice and wild type (WT) mice were procured from Shanghai Model Organisms Center in Shanghai, Inc. (License No. SCXK (Yu) 2020-0005).

Techniques: Knock-Out, Expressing, Immunofluorescence, Staining, Control, Fluorescence, TUNEL Assay

Journal: Neural Regeneration Research

Article Title: Low-level expression of Cmyc in mature neurons: Maintaining neuronal function and preventing neurodegeneration

doi: 10.4103/NRR.NRR-D-24-01367

Figure Lengend Snippet: Cmyc knockout in midbrain dopamine neurons for 60 days decreases the expression levels of DAT and TH (western blot analysis). (A) Expression levels of D1R, D2R, DAT in the striatum and TH in the substantia nigra 60 days after Cmyc knockout in midbrain dopamine neurons. (B–E) Quantification of the expression levels of D1R (B) and D2R (C), DAT (D), and TH (E). * P < 0.05, ** P < 0.01, vs. WT (one-way analysis of variance followed by Tukey’s post hoc test). Data are presented as the mean ± SEM ( n = 3). D1R: Dopamine D1 receptor; D2R: dopamine D2 receptor; DAT: dopamine transporter; ns: not significant; KO: knockout; TH: tyrosine hydroxylase; WT: wild-type.

Article Snippet: Cmyc flox/– heterozygous mice and wild type (WT) mice were procured from Shanghai Model Organisms Center in Shanghai, Inc. (License No. SCXK (Yu) 2020-0005).

Techniques: Knock-Out, Expressing, Western Blot

Journal: Neural Regeneration Research

Article Title: Low-level expression of Cmyc in mature neurons: Maintaining neuronal function and preventing neurodegeneration

doi: 10.4103/NRR.NRR-D-24-01367

Figure Lengend Snippet: High-throughput sequencing results show that Cmyc knockout in midbrain dopaminergic neurons causes neuronal senescence. (A) Horizontal histogram displaying GO Enrichment top 20 pathways of upregulated DEGs of Cmyc KO groups compared with control groups. (B, C) Scatter plots of the top 20 KEGG enrichment of upregulated (B) and downregulated (C) DEGs of Cmyc KO groups compared with control groups. (D) Volcano plot displays DEGs of Cmyc KO groups compared with control groups. Significantly upregulated genes are colored red, significantly downregulated genes are colored blue. (E) The upregulated cytokines (red) of Cmyc KO groups in KEGG pathways. (F–H) Enrichment score of the microglia activation pathway (F), respiratory electron transport pathway (G), and oxidative damage response pathway (H) in Cmyc KO groups versus control groups analyzed by GSEA. n = 3. DEGs: Differentially expressed genes; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; KO: knockout.

Article Snippet: Cmyc flox/– heterozygous mice and wild type (WT) mice were procured from Shanghai Model Organisms Center in Shanghai, Inc. (License No. SCXK (Yu) 2020-0005).

Techniques: Next-Generation Sequencing, Knock-Out, Control, Activation Assay

Journal: Neural Regeneration Research

Article Title: Low-level expression of Cmyc in mature neurons: Maintaining neuronal function and preventing neurodegeneration

doi: 10.4103/NRR.NRR-D-24-01367

Figure Lengend Snippet: Cmyc knock out facilitates neuronal aging and nitro-α-syn aggregation. (A) At 60 days after Cmyc knockout ( n = 3) in substantia nigra dopaminergic neurons, immunofluorescence staining in the substantia nigra region showed aggregation of nitro-α-syn. The green fluorescence indicated by the arrow is the neuraxond spheroid. Scale bars: 200 μm for low-magnification images and 25 μm for high-magnification images. (B) At 60 days after Cmyc knockout ( n = 3), SA-β-gal staining in the substantia nigra region showed a deep blue product. Scale bars: 200 μm for low-magnification images and 40 μm for high-magnification images. Regions highlighted in yellow are the substantia nigra site. (C) Klotho expression in the substantia nigra region 60 days after Cmyc knockout ( n = 3). (D) The mRNA expression of CXCR4 , CCL5 , and P21 expression levels in the substantia nigra dopaminergic neurons 60 days after Cmyc knockout ( n = 3). (E) In PC9 cells with c-MYC knockout, SA-β-gal staining showed a deep blue product compared with that in wild-type PC9 cells. Scale bar: 25 μm. (F, G) The mRNA expression of klotho (F) and CCL5 , P21 , and CXCR4 (G) in PC9 cells after c-MYC knockout. Data are presented as the mean ± SEM. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance followed by Tukey’s post hoc test). KO: Knockout; WT: wild-type.

Article Snippet: Cmyc flox/– heterozygous mice and wild type (WT) mice were procured from Shanghai Model Organisms Center in Shanghai, Inc. (License No. SCXK (Yu) 2020-0005).

Techniques: Knock-Out, Immunofluorescence, Staining, Fluorescence, Expressing